H. Welzel, G. Kossmehl, G. Engelmann
1998
Citations
0
Influential Citations
2
Citations
Journal
Macromolecular Symposia
Abstract
The hydroxy group of 3-(2-hydroxyethyl)thiophene was protected as methyl ether I and as dimethyl tert-butyl silyl ether 5 before anodic polymerization. The poly[3-(2-methoxyethyl)thiophene] 2 was prepared by electrochemical homopolymerization of 1. Ether cleavage was carried out in the polymer film 2 and the resulting poly[3-(2-hydroxyethyl)thiophene) (3) was activated with cyanogen bromide to immobilize alcohol dehydrogenase. Silylether 5 did not undergo homopolymerization but copolymerization of 5 with 3-methylthiophene (4) was successful. After cleavage of the protecting group the resulting copolymer 7 was activated by cyanuric chloride, and chymotrypsin was immobilized Electrocopolymerization of thiophene-3-acetic acid (8) and 3-methylthiophene (4) under various conditions produces copolymer 9. By activation of the carboxylic groups with N,N'-dicyclohexylcarbodiimide (DCC) lactate oxidase (LOD) was bond to the surface of the electrode to form a lactate sensor.