K. Sasamoto, T. Ushijima, M. Saito
Apr 1, 1996
Citations
1
Influential Citations
19
Citations
Journal
Analytical Sciences
Abstract
A new coumarin-based amine, 4-aminomethyl-6,7-dimethoxycoumarin, was synthesized as a precolumn fluorescence derivatization reagent for carboxylic acids. The derivatization using the amine in a two-phase medium of water and ethyl acetate was examined. The two-phase derivatization method with the amine was found to be of value since it allows simple and rapid HPLC assays of carboxylic acids with a reasonable sensitivity and separability. The detection limit was determined to be 20–50 fmol per 10-μl injection volume at a signal-to-noise ratio of 3 under partially optimized conditions. The method was also applied to the assay of fatty acids in human serum.