Wu Hua-che
2013
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Quality indicators
Journal
Chinese Journal of Synthetic Chemistry
Abstract
Two immunogens(1 and 2) were prepared by coupling of 5-methylsalicylic acid with BSA through a combination method of active ester and EDC or a mixed-anhydride method. The coupling ratios of 1 and 2 are 14. 6 and 3. 4,respectively. Two antibodies(3 and 4) were obtained by the stimulating male New Zealand rabbit with 1 or 2. 3 and 4 were characterized by i-Elisa methods. The results showed that the titer of 3 and 4 were 1 ∶ 256 000 and 1 ∶ 128 000. An i-Elisa method for 5-methylsalicylic was established with 3,and the results indicated that IC50was 149. 04 ng·mL-1and LOD was 4. 19 ng·mL-1. The cross-reactivity rates of 3 with 2-amino-p-cresol,phenol and cresol were all less than 0. 1%.