R. A. Haack, P. Macdonald, Q. Ruan
Jul 21, 2020
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Journal
Tetrahedron Letters
Abstract
Abstract We describe the preparation and utility of fluorescent heterobifunctional probes derived from an easily prepared bifunctional rhodamine core possessing carboxylic and sulfonic acid functional groups. Trichloroacetonitrile/PPh3 was employed as a mild chlorinating reagent for this complex system to allow functionalization of the sulfonic acid. This technique is described as an alternative to harsh chlorinating conditions. The synthesized probes allow for the introduction of functionality (ie. biotin) to biomolecules and direct quantification of incorporation through absorbance or fluorescence measurements.