O. Adachi, Y. Ano, H. Toyama
Dec 23, 2006
Citations
0
Influential Citations
13
Citations
Journal
Bioscience, Biotechnology, and Biochemistry
Abstract
A method for enzymatic preparation of 3-dehydroquinate and 3-dehydroshikimate in the shikimate pathway was established by controlling the enzyme activity of 3-dehydroquinate dehydratase. When quinate was incubated with the membrane fraction of acetic acid bacteria at pH 5.0, 3-dehydroquinate was formed as the predominant product. 3-Dehydroshikimate was the sole product when incubated at pH 8.0. Mutual separation of the metabolic intermediates was also exemplified.