C. Krog-Jensen, M. Christensen, M. Meldal
Jul 1, 1999
Citations
0
Influential Citations
1
Citations
Journal
Letters in Peptide Science
Abstract
A new and facile synthesis of tyrosine phosphorylated peptides has been developed.N α-Fmoc-Tyr(tBu)-OPfp was treated with TFA, phosphorylated with phosphorous oxychloride and the resulting phosphoric acid dichloride was hydrolysed to giveN α-Fmoc-Tyr(PO3H2)-OPfp1 in an overall yield of 98%. Compound1 was used in solid-phase peptide synthesis of phosphopeptides2, 3 and4, which are fragments of murine adipocyte lipid binding protein. The advantage of using the Pfp ester was the absence of pyrophosphates and other byproducts.