B. Erlanger, Nicholas Kokowsky, William W. Cohen
Nov 1, 1961
Citations
154
Influential Citations
3,036
Citations
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Journal
Archives of biochemistry and biophysics
Abstract
Abstract The synthesis and properties of l -lysine p -nitroanilide dihydrobromide and benzoyl dl -arginine p -nitroanilide hydrochloride are described. Both compounds are hydrolyzed by trypsin, the latter being a substrate at least as sensitive as benzoyl l -argininamide. Their hydrolysis can be followed conveniently by colorimetric procedures, since one of the products, p -nitroaniline, is yellow. Values of K m and k 3 for their reaction with trypsin were determined, as well as the K 4 of benzoyl d -arginine p -nitroanilide, which was isolated from a tryptic digest of the dl isomer. The pH-activity curves were also determined, that of l -LPA being in a more alkaline region than normally found for trypsin substrates. The possible significance of this shift is discussed. Preliminary studies indicate that benzoyl dl -arginine p -nitroanilide hydrochloride is also hydrolyzed by papain.