C. Ballou
1963
Citations
0
Influential Citations
15
Citations
Journal
Methods in Enzymology
Abstract
Publisher Summary This chapter describes the preparation and properties of D -erythrose 4-phosphate. A preparation procedure involves the phosphorylation of a suitable blocked derivative of D -erythrose, leading to D -erythrose 4-phosphate dimethyl acetal. Free D -erythrose 4-phosphate is obtained by mild acid hydrolysis of the acetal. Another procedure is a one-step synthesis carried out by the lead tetraacetate oxidation of D -glucose 6-phosphate. D -Erythrose 4-phosphate is similar to glyceraldehyde 3-phosphate in its sensitivity to acid, the half-time for hydrolysis with the formation of inorganic phosphate in 1 N acid at 100° being 20 minutes. On the other hand, treatment with 1 N sodium hydroxide at room temperature results in the rapid but incomplete elimination of phosphate as inorganic phosphate. D -erythrose 4-phosphate is oxidized slowly by D-glyceraldehyde 3-phosphate dehydrogenase, and a method for its estimation is based on this fact. It is also rapidly condensed with dihydroxyacetone phosphate in the presence of rabbit muscle aldolase to yield sedoheptulose diphosphate and with phosphoenolypyruvate in the presence of enzymes from Escherichia coli to yield dehydroshikimic acid.