P. Taberner, J. Barnett, G. A. Kerkut
Jan 1, 1972
Citations
0
Influential Citations
17
Citations
Journal
Journal of Neurochemistry
Abstract
Abstract— A simple method is described for the preparation of succinic semialdehyde by the oxidation of 4‐hydroxybutyric acid with dimethyl sulphoxide in the presence of phosphorus pentoxide. The colorimetric estimation of succinic semialdehyde by condensation with 3‐methyl‐2‐benzothiazolinone‐2‐hydrazone is used as an assay for GABA aminotransferase activity in mouse brain. The values obtained for the specific activity of this enzyme in mouse brain using the colorimetric method agree with those obtained by the more conventional isotopic method.