Yan Li, Junqing Wang, Fen Wang
Nov 18, 2021
Citations
0
Influential Citations
4
Citations
Journal
ChemSusChem
Abstract
10-Hydroxy-2-decenoic acid (10-HDA) is a terminal hydroxylated medium-chain α, β-unsaturated carboxylic acid that performs various unique physiological activities and has a wide market value. Therefore, development of an environmentally friendly, safe, and high-efficiency route to synthesize 10-HDA is required . Here, we modified the β-oxidation pathway of Escherichia coli and rationally designed P450 terminal hydroxylase (CYP153A33-CPR BM3 ) to synthesize 10-HDA using decanoic acid as a substrate via two-step whole-cell catalysis. We analysed different homologues of FadDs, FadEs, and YdiIs in the first step of the conversion of decanoic acid to trans- 2-decenoic acid. In the second step, CYP153A33 (M228L)-CPR BM3 efficiently catalysed the conversion of trans -2-decenoic acid to 10-HDA. Finally, we obtained 217 mg L -1 10-HDA with 500 mg L -1 decanoic acid. Our study provides a strategy for biosynthesis of 10-HDA and other α, β-unsaturated carboxylic acid derivatives from specific fatty acids.