Wenqiang Ren, D. Cai, Song Hu
Nov 1, 2017
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Influential Citations
8
Citations
Journal
Process Biochemistry
Abstract
Abstract This study sought to develop a cost-effective biological catalysis process for S -adenosyl- l -methionine (SAM) production. During the process, a mixture of d -methionine ( d -Met) and l -methionine ( l -Met), namely dl -Met, was used as substrate to replace the conventional but expensive pure l -Met. The concentration of dl -Met in substrate was optimized. When 80 g/L of dl -Met was added in a 5 L-scale bioreactor, 13.74 g/L of SAM was produced with an l -Met conversion rate of 32.15%. The fermentation process was then scaled up to meet the requirements of realistic industrial SAM production. In a 300 L-scale fermentation process, 10.45 g/L of SAM was achieved, with an l -Met conversion rate of 32.61%. Moreover, the proportion of d -Met remaining in broth increased from 50% at the beginning to 76.89% at the end of fermentation. The fermentation process is generally appropriate for commercial SAM production.