Chang-Jing G. Yeh, Bae-Li Hsi, W. Faulk
Jun 30, 1981
Citations
3
Influential Citations
200
Citations
Quality indicators
Journal
Journal of immunological methods
Abstract
The use of a fluorescence method employing propidium iodide to determine nuclear morphology of different cell types by epi-illumination in membrane immunofluorescence is described. Its usefulness is illustrated by differentiating nucleated and anuclear transferrin receptor-bearing cells as well as by simultaneously determining the viability of cell suspension without requiring a change from fluorescence microscopy, this causing no loss of visual accommodation.