M. Carpenter
Jun 1, 1974
Citations
1
Influential Citations
14
Citations
Quality indicators
Journal
Lipids
Abstract
The purpose of the study was to determine whether prostaglandins were present in mammalian testis, a tissue that has a large concentration of polyenoic fatty acids that are potential precursors of prostaglandins. Acid-soluble lipids of rat testis were extracted, purified, and fractionated by thin layer and column chromatographies.3H-Prostaglandins were added as internal reference standards to monitor recoveries and facilitate identification. Initial identification of prostaglandin species was done by chromatography. Further identification was done by elution of the prostaglandin zones followed by rechromatographies (both thin layer and column), measurements of UV absorption spectra, and by gas liquid chromatography. The results of these analyses indicate that prostaglandin E1, 11α,15(S)-dihydroxy-9-oxo-β-trans-prostenoic acid; prostaglandin E2, 11α-15(S)-dihydroxy-9-oxo-5-cis-13-trans-prostadienoic acid; and prostaglandin F1, 9α,11α,15(S)-trihydroxy-13-trans-prostenoic acid occur in rat testicular tissue and that prostaglandin F2α, 9α, 11α, 15(S)-trihydroxy-5-cis-13-trans-prostadienoic acid and prostaglandin E2, 11α,15(S)-dihydroxy-9-oxo-5-cis-13-trans-prostadienoic acid may be the primary species of this tissue. Prostaglandin B1, 15(S)-hydroxy-9-oxo-8(12),13-trans-prostadienoic acid and prostaglandin B2, 15(S)-hydroxy-9-oxo-5-cis,8(12),13-trans-prostatrienoic acid also were detected, and some evidence was obtained for the presence of prostaglandin metabolites.