Anthony G. Frutos, and Jennifer M. Brockman, R. Corn
Mar 1, 2000
Citations
0
Influential Citations
66
Citations
Journal
Langmuir
Abstract
The reversible protection of amine- and hydroxyl-terminated alkanethiol self-assembled monolayers (SAMs) on gold surfaces using the base-labile protecting group 9-fluorenylmethoxycarbonyl (Fmoc) and the acid-labile protecting group dimethoxytrityl (DMT) is described. When used in combination with UV photopatterning or μ-contact printing techniques, this reversible SAM protection chemistry can be used to control the reactivity and wettability of different portions of the surface. Such spatial control is utilized in the fabrication of DNA arrays. Specifically, gold surfaces modified with the amine-terminated alkanethiol 11-mercaptoundecylamine (MUAM) are reacted with an N-hydroxysuccinimide (NHS) ester derivative of Fmoc, forming a covalent urethane (carbamate) linkage and converting the initially hydrophilic MUAM surface to a hydrophobic, Fmoc-terminated surface. Upon exposure of the Fmoc-modified surface to a solution of a secondary amine, the Fmoc is cleaved, regenerating the original hydrophilic MUAM su...