A. Gilman
Sep 1, 1970
Citations
28
Influential Citations
2,781
Citations
Quality indicators
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abstract
A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10(-9) M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.