C. Deswarte, Sandrine Peyrebrune, H. Canut
Aug 19, 2015
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Journal
Vitis: Journal of Grapevine Research
Abstract
Introduction: Eutypiosis, due to the ascomycete fungus Eutypa lata, affects a number of vineyards in the world. We have demonstrated that the pathogenic agent synthesises a toxin, 4-hydroxy-3-[3-methyl-3-butene-1ynyl] benz-aldehyde (RENAUD et al. 1989), named eutypine, which participates in the pathogenic action of Eutypa lata (TEY-RULH et al. 1991, PHILIPPE et al. 1992). The aim of our research is to determine the mechanism of action of eutypine. Its primary target can be located on the plasma membrane or into the cells. In both cases, an interaction between the toxin and the plasma membrane must necessarily occur. Also, we have developed an efficient method to obtain highly purified plasma membrane vesicles from Vitis vinifera cells, using free-flow electrophoresis.