R. Lotan, A. Gussin, H. Lis
May 15, 1973
Citations
0
Influential Citations
71
Citations
Quality indicators
Journal
Biochemical and biophysical research communications
Abstract
Abstract Sepharose-2-acetamido-N-(ϵ-aminocaproyl)-2-deoxy-β-D-glucopyranosylamine was prepared by a reaction of 2-acetamido-3,4,6-tri-0-acetyl-2-deoxy-β-D-glucopyranosylamine and N-(benzyloxycarboxyl)-ϵ-aminocaproic acid, removal of the 0-acetyl and the benzyloxycarboxyl groups and coupling to Sepharose. The product was used for the purification of wheat germ agglutinin, by adsorption from a crude wheat germ extract and elution with 0.1M acetic acid. The purified agglutinin was homogeneous on SDS-polyacrylamide gel electrophoresis and had a specific hemagglutinating activity of 3000 u/mg when tested on trypsinized rabbit erythrocytes. It was rich in cysteine, cystine and glycine, and contained no sugar.