M. J. Nozal, J. Bernal, V. Hernández
Apr 1, 1993
Citations
0
Influential Citations
7
Citations
Journal
Journal of Liquid Chromatography & Related Technologies
Abstract
Abstract A comprehensive study of the reaction between Purpald (4-amino-3- hydrazino-5-mercapto-l, 2, 4-triazole) and eight neutral monosaccharides in order to establish the optimal conditions (wavelength, oxygen supply, temperature, time, pH and concentration range) was carried out. The reaction was applied to the post-column detection in HPLC of monosaccharides eluted with water from an ion-exchange column. The detection limits and linear determination ranges thus achieved make this reaction a valid alternative to the determination of carbohydrates.