A. Vahidi, Henry R Roberts, Joseph San Filippo
Sep 1, 1971
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0
Influential Citations
13
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Journal
Clinical chemistry
Abstract
We describe a quantitative paper-chromatographic method for assaying 3-methoxy-4-hydroxymandelic acid (VMA) in urine. VMA is extracted from acidified urine into ethyl acetate, separated from interfering amine, acid, and alcoholic metabolites by paper chromatography, and reacted with diazotized p -nitroaniline. The colored derivative is eluted and spectrophotometrically analyzed. Recoveries of replicates of standard and sample solutions on the same chromatogram exceeded 95%.