Ibrahim Khambati, Sang-Gyu Han, Daniëlle Pijnenburg
Nov 28, 2016
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0
Influential Citations
7
Citations
Quality indicators
Journal
Inflammation Research
Abstract
ObjectiveBacterial colonization relies on communication between bacteria via so-called “quorum-sensing molecules”, which include the acyl-homoserine lactone group. Certain acyl-homoserine lactones can modulate mammalian cell function and are thought to contribute to bacterial pathogenicity. Given the role of mast cells in host defense, we investigated the ability of acyl-homoserine lactones to modulate mast cell function.MethodsWe utilized murine primary mast cell cultures to assess the effect of acyl-homoserine lactones on degranulation and cytokine release in response to different stimuli. We also assessed cell migration in response to chemoattractants. The effect of acyl-homoserine lactones in vivo was tested using a passive cutaneous anaphylaxis model.ResultsTwo of the tested quorum-sensing molecules, N-3-oxo-dodecanoyl-l-homoserine lactone and N-Dodecanoyl-l-homoserine lactone, inhibited IgE dependent and independent degranulation and mediator release from primary mast cells. Further testing of N-3-oxo-dodecanoyl-l-homoserine lactone, the most potent inhibitor and a product of Pseudomonas aeruginosa, revealed that it also attenuated chemotaxis and LPS induced cytokine production. In vivo, N-3-oxo-dodecanoyl-l-homoserine lactone inhibited the passive cutaneous anaphylaxis response in mice.ConclusionThe ability of N-3-oxo-dodecanoyl-l-homoserine lactone to stabilize mast cells may contribute to the pathogenicity of P. aeruginosa but could potentially be exploited therapeutically in allergic disease.