A. Cashmore, D. M. Brown, J. Smith
Jul 28, 1971
Citations
0
Influential Citations
48
Citations
Journal
Journal of molecular biology
Abstract
Abstract The effect of pH on the rate and product ratio has been investigated for the reaction of methoxyamine with cytidine. In the pH range 5.0 to 5.5, approximately 20% of the cytosine bases in mixed Escherichia coli transfer RNA are reactive towards methoxyamine. The cytosine bases in the su + III tyrosine suppressor tRNA which are reactive towards methoxyamine have been characterized as C-16, 19, 35, 51, 83 and 84. Surprisingly, residue C-33, a base at the 5′ terminal of the anticodon loop, is relatively resistant to modification. These results are discussed in terms of transfer RNA conformation.