J. Hardman, D. Hardman
Nov 10, 1971
Citations
0
Influential Citations
9
Citations
Journal
The Journal of biological chemistry
Abstract
Abstract 1,5-Difluoro-2,4-dinitrobenzene introduces an intramolecular cross-linkage into the Escherichia coli tryptophan synthetase α subunit between residues cysteine 80 and lysine 108, concomitant with a loss of its aldolytic activity on indoleglycerol phosphate. The stereochemical relationship of these residues and other residue pairs cross-linked by other bifunctional reagents to the activity of the enzyme is discussed.