H. Noritomi, A. Watanabe, H. Kise
Feb 1, 1989
Citations
0
Influential Citations
15
Citations
Journal
Polymer Journal
Abstract
Abstractα-Chymotrypsin (CT) was immobilized to poly(vinyl alcohol) (PVA) by adsorption from its aqueous solutions. The catalytic activity of CT increased markedly by immobilization for peptide or ester synthesis from N-acetyl-l-tyrosine in hydrophilic organic solvents such as acetonitrile or ethanol. The yields of the peptide and ester are strongly dependent on the PVA/CT ratio and water content in the reaction medium. The rate and equilibrium constant of the ester formation reaction are also dependent on water content. These results are discussed in terms of the activation and stabilization of CT in hydrous PVA matrix. The studies on the substrate- and stereo-selectivity for the ester formation reactions suggest that CT maintains its native conformation in PVA matrix. The stability of PVA-immobilized CT is also described.