G. Sabbioni, J. Lamb, P. Farmer
1997
Citations
0
Influential Citations
3
Citations
Journal
Biomarkers
Abstract
Arylisocyanates are important intermediates in the chemical industry. Amongst the main damage after low levels of isocyanate exposure are lung sensitization and asthma. Protein adducts of isocyanates might be involved in the aetiology of sensitization reactions. Blood protein adducts are used as dosimeters for modifications of macromolecules in the target organs where the disease develops. To develop methods for the quantitation of protein adducts we reacted 4 methylphenyl isocyanate 4MPI with the tripeptide valyl glycyl glycine and with single amino acids yielding N 4 methylphenyl carbamoyl L valyl glycyl glycine 4MPI Val Gly Gly, N 4 methylphenyl carbamoyl L valine 4MPI Val, N 4 methylphenyl carbamoyl L aspartic acid 4MPI Asp, N acetyl S 4 methylphenyl carbamoyl L cysteine 4MPI AcCys, N acetyl N 4 methylphenyl carbamoyl lysine 4MPI AcLys, N acetyl O 4 methylphenyl carbamoyl tyrosine 4MPI AcTyr and N acetyl O 4 methylphenyl carbamoyl D,L serine 4MPI AcSer. The hydrolysis of the adducts was tested under a...