M. Seraydarian, M. Seraydarian, L. Artaza
Sep 1, 1976
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Influential Citations
39
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Journal
Journal of molecular and cellular cardiology
Abstract
Abstract Cardiac and skeletal muscle cells grown in culture were used in experiments designed to test the regulatory function of creatine. An increased concentration of intracellular phosphorylcreatine resulted from the addition of creatine to the growth media and the addition of metabolic inhibitors prevented the new high steady state concentration without depletion of ATP. With 1-fluoro-2, 4-dinitrobenzene the high concentration of phosphorylcreatine remained unchanged, while ATP was depleted. The use of the inhibitors provided additional evidence for creatine as the effector molecule in a feedback regulation of energy production, and for phosphorylcreatine in the regulation of energy charge at the contractile site. The effect of creatine on the fusion of myoblasts into myotubes supported its role in the control of cellular functions other than those involved in the energy metabolism. The correlation of the growth of muscle with increased muscular activity might be mediated through creatine, the end product of muscle activity. Creatine, synthesized in tissues other than muscle, might be involved in the coordination of muscle development throughout the body. Furthermore, it is suggested that the metabolic regulatory function of creatine is limited to the cells which do not synthesize creatine de novo , while in the tissues, such as the liver and kidney which have the mechanism for de novo synthesis of creatine, the absence of mitochondrial CPK precludes metabolic regulation of ∼ P synthesis by creatine.