M. Schwochau, L. Hadwiger
Oct 1, 1969
Citations
1
Influential Citations
46
Citations
Journal
Archives of biochemistry and biophysics
Abstract
Abstract Pisatin induction in pods of Pisum sativum L. is dependent on newly synthesized protein and RNA. Cycloheximide (300 μg/ml) and actinomycin D (450 μg/ml) completely inhibit the synthesis of pisatin if added with the inducer. Compounds [actinomycin D, chromomycin A3, mitomycin C, N-methyl-N′-nitro-N-nitrosoguanidine (nitrosoguanidine), di-(2-chloroethyl) methylamine-HCl (nitrogen mustard), and 8-azaguanine] known to react with, change or substitute for the guanine moiety of double stranded DNA are at certain concentrations potent inducers of pisatin production. Acridine orange and ethidium bromide which can intercalate into double-stranded DNA also induce pisatin production. In addition to the stimulation of pisatin production, actinomycin D (10 μg/ml) also stimulates the rate of total protein and RNA synthesis. A mechanism for gene activation based on changes in the conformation of regulatory sites on double-stranded DNA is proposed.