D. Blond, R. Whittam
Jul 1, 1964
Citations
0
Influential Citations
78
Citations
Quality indicators
Journal
The Biochemical journal
Abstract
Adenosine-triphosphatase activity was measured in the same homogenate after diluting tenfold and allowing the coarse fibres to settle out. The reaction mixture contained MgCl2 (2 mm), ATP (disodium salt, buffered to pH 7-8 with tris) (2 mM), imidazole-HCl buffer, pH 7-8 (15 mM), and the appropriate concentrations of NaCl, KCI and ouabain. The amount of orthophosphate liberated was determined after incubating for 5 min. at 37°. All points are means of duplicate determinations. The Figure illustrates two experiments typical of four such experiments.