Zheng Lin-xin
2015
Citations
0
Influential Citations
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Citations
Quality indicators
Journal
Chinese Journal of Birth Health & Heredity
Abstract
Objective:Discussion of β calcitonin gene(Human beta-CGRP gene exon 4 for calcitonin-like peptide,CGRP)the relationship between polymorphism and serum CGRP peptide level and the development of type 1 diabetes. Methods:Polymerase chain reaction(polymerase chain reaction,PCR),42 cases of type 1diabetic patients and 126 normal control class β calcitonin gene full-length amplification and genotyping,gene frequency statistics of type 1 diabetes group and normal control group beta calcitonin gene type,at the same time enzyme linked immunosorbent assay(enzyme-linked immunosorbent assay,ELISA)were determined by difference of calcitonin gene related peptide concentration in each category of each genotype serum. Results:Univariate Logistic regression analysis showed that beta calcitonin gene polymorphism(155209 C)are associated with the risk of type 1 diabetes,the correlation coefficients were 3.27,the concentration of serum CGRP of patients with type 1diabetes with normal control group 1.40 times. Conclusions:Calcitonin gene polymorphism and genetic susceptibility to type 1diabetes,serum CGRP concentration can be used as a novel marker for type 1 diabetes.