M. Maurs, F. Acher, R. Azerad
Dec 1, 2012
Citations
0
Influential Citations
4
Citations
Journal
Journal of Molecular Catalysis B-enzymatic
Abstract
Abstract In order to deprotect N -carbobenzoxy- l -aminoacids (Cbz-AA) and related compounds, a series of microorganisms was selected from soil by enrichment cultures with Cbz- l -Glu as sole nitrogen source. A lyophilized whole-cell preparation of two Arthrobacter sp. strains grown on Cbz-Glu or Cbz-Gly exhibited a high cleavage activity. The conditions of hydrolysis have been optimized and a quantitative enantioselective deprotection of several Cbz- dl -amino acids was obtained, as well as the deprotection of N -carbamoylester derivatives of several synthetic amino compounds. The preparation of Cbz- d -allylglycine and l -allylglycine in high yield and high optical purity is described as an application of this method.