F. Amaldi, F. Carnevali, L. Leoni
Oct 1, 1972
Citations
1
Influential Citations
70
Citations
Quality indicators
Journal
Experimental cell research
Abstract
Abstract Fluorodeoxyuridine and aminopterin, commonly used to synchronize animal cells at the beginning of S phase, are shown to be unsuitable for studying the origins of replicons because of their unsatisfactory action as DNA synthesis inhibitors, whereas hydroxyurea is suitable for this purpose. Moreover, the results presented support the bidirectional model for DNA replication in animal cells and suggest that the timing of initiation of various origins is independent of the rate of synthesis at each replication fork.