Li We
2014
Citations
0
Influential Citations
2
Citations
Quality indicators
Journal
China Journal of Modern Medicine
Abstract
【Objectives】To analysis the inhibit proliferation and induced apoptosis effects of 1-Methylhydantoin in human colonic cancer cell line SW480. 【Methods】Cultivated human colon cancer SW480 cells in vitro as the study abject, MTT assay was used to detect the absorbance after treatment with 1-Methylhydantoin at different concentrations(0, 0.5, 5, 10, 20, 50, 100, 200, 500 mg/L) alone or in combination with 5-fluorouracil at different concentrations(10, 20, 50, 100 mg/L) on the proliferation of SW480 cells for different time(24 h, 48 h, and 72 h), then the inhibition ratio on SW480 cells was derived. 1-Methylhydantoin and 5-fluorouraci with a special concentration(100 mg/L) respectively effect on SW480 cells ordinary microscope. 【Results】MTT assay results of proliferation inhibitory rate in experimental groups show that: 1-Methylhydanto could suppress proliferation of SW480 cells within the range of experimental concentrations(0.5, 5, 10, 20, 50, 100, 200, 500 mg/L), and the function intension depended on the density of 1-Methylhydantoin(P 0.05). 1-Methylhydantoin exhibited a highest inhibition ratio on colonic cancer cell line SW480 after effecting 48 hour.【Conclusions】1-Methylhydantoin could inhibit proliferation and induced apoptosis of colon cancer SW480 cells in vitro, blocked cell cycle in G0/G1 phase.