Erhua Zhang, Xiaoping Chen, X. Liang
Mar 1, 2011
Citations
1
Influential Citations
13
Citations
Journal
ELECTROPHORESIS
Abstract
In this short communication, a novel protocol for resolubilization of TCA‐precipitated plant proteins for 2‐DE is described. Guanidine hydrochloride (Gdn‐HCl) is used as an intermediate for protein solubilization in which proteins are reduced and alkylated with tributylphosphane (TBP) and 2‐vinylpyridine (2‐VP). The blocking of –SH groups at Cys residues can greatly improve the solubility of TCA‐precipitated proteins and obtain more high‐quality protein spots in the 2‐DE gel. This protocol is compatible with silver stain and MS identification.