K. Otsuka, S. Terabe
Aug 31, 1990
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Journal
Journal of Chromatography A
Abstract
Abstract Enantiomeric resolution of phenylthiohydantoin (PTH)-amino acids by micellar electrokinetic chromatography using chiral surfactants was studied. As a chiral surfactant, digitonin, which is a non-ionic compound, was used with anionic sodium dodecyl sulphate (SDS) to form mixed micelles. Under acidic conditions (pH 3.0), PTH derivatives of six amino acids (tryptophane, norleucine, norvaline, valine, α-aminobutyric acid and alanine) were separated from each other and optically resolved with a 25 m M digitonin-50 m M SDS solution, although a long separation time was required. The use of an anionic chiral surfactant, sodium N-dodecanoyl- l -valinate, was also examined under neutral conditions. In this instance, the same enantiomers as above except PTH- dl -Ala were resolved in a shorter time.