R. T. Krause
Dec 20, 1979
Citations
0
Influential Citations
77
Citations
Quality indicators
Journal
Journal of Chromatography A
Abstract
Abstract N-Methyl carbamate insecticides and carbamate metabolities are separated on octylsilane and cyanopropylsilane bonded packing with an acetonitrile-water gradient mobile phase. The eluted carbamates are detected at the nanogram and sub- nanogram levels with a fluorescence detector after in-line hydrolysis to methylamine and subsequent reaction with o -phthalaldehyde and 2-mercaptoethanol to form the fluorophore. Using this high-performance liquid chromatographic (HPLC) technique, no responce was observed for 182 pesticides including various amides, anilides, anilines, carboximides, N-methyl carbamates, triazines, ureas and other nitrogen-containing pesticides. The HPLC post-column fluoremetric system provides the resolution, sensitivity and selectivity desired for the determination of multicarbamate insecticide residues.