Nichols Jw, Pagano Re
Apr 13, 1982
Citations
0
Influential Citations
87
Citations
Quality indicators
Journal
Biochemistry
Abstract
Resonance energy transfer between 7-nitro- 2,1,3-benzoxadiazol-4-y1 (NBD) acyl chain labeled lipids (the energy donors) and (lissamine) Rhodamine B labeled phos- phatidylethanolamine (N-Rh-PE) (energy acceptor) was used to monitor the rate of transfer of NBD-labeled lipids between two populations of small vesicles. When both fluorescent lipid analogues were incorporated into the same donor vesicle at - 1 mol %, NBD fluorescence was quenched by the N-Rh-PE due to resonance energy transfer. Addition of nonfluorescent acceptor vesicles to these donor vesicles resulted in an im- mediate and continuous change in NBD fluorescence due to the transfer of the NBD lipids, but not the nonexchangeable N-Rh-PE (Pagano, R. E., Martin, 0. C., Schroit, A. J., & P-C6-NBD-PC, 1 -palmitoyl-2- (6-((7- nitro-2,1,3-benzoxadiazol-4-yl)amino)caproyl)phosphatidylcholine; P- C6-NBD-PA, 1-palmitoyl-2-(6-((7-nitro-2,1,3-benzoxadiazol-4-y1)- amino)caproyl)phosphatidic acid; P-C6-NBD-POA, 4-palmitoyl-3-(6- ( (7-nitro-2,1,3-benzoxadiazol-4-yl)amino)caproyl)oxybutyl- 1-phosphonic acid; C6-NBD-PC, 1-acyl-2-(6-((7-nitro-2,1,3-benzoxadiazol-4-yl)- amino)caproyl)phosphatidylcholine; C6-NBD-PA, l-acyl-2- (6- ((7-nitro- 2,1,3-benzoxadiazol-4-yl)aminojcaproyl)phosphatidic acid; C6-NBD-PE, 1 -acyl-2- (6- ( (7-nitro-2,1,3-benzoxadiazol-4-yl)amino)caproyl) phospha- tidylethanolamine; C6-NBD-PG, l-acyl-2-(6-((7-nitro-2,1,3-benz- oxadiazol-4-yl)amino)caproyl)phosphatidylglycerol; C6-NBD-DG, 1- acyl-2-(6-((7-nitro-2,1,3-benzoxadiazol-4-yl)amino)caproyl)diglyceride; C,,-NBD-PC, l-acyl-2- ( 12- ((7-nitro-2,1,3-benzoxadiazol-4-yl)amino)-