Guofang Zeng, Fengping Chen, Yating Lei
Sep 9, 2021
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Influential Citations
2
Citations
Quality indicators
Journal
Luminescence : the journal of biological and chemical luminescence
Abstract
Resorcinol (1,3-dihydroxybenzene) is a common coupling agent in permanent hair dyes, which has arrested people's attention for its potential hazard to the human health. However, the action mechanism of resorcinol and human DNA has not been elucidated yet. In this research, the binding property between resorcinol and calf thymus DNA (ct-DNA) was first studied through various spectral and molecular docking techniques. Spectral studies showed that the initial fluorescence quenching of resorcinol against DNA was a static one. The result of ΔH < 0 and ΔS > 0 was produced from thermodynamic experiment data, thus it could be concluded that electrostatic force was the major driving force, while the binding constant Kb was 1.56×104 M-1 at 298 K. The electrostatic binding network between resorcinol and ct-DNA was established explicitly through competitive substitution analysis and other spectral approaches. The result of FT-IR absorption spectra indicated resorcinol had bound to DNA phosphate skeleton. Molecular docking clearly revealed the binding occurred between hydroxyl groups of resorcinol and phosphorus oxygen bonds (P-O) of the DNA skeleton. These findings may deepen our understanding of the action mechanism between resorcinol and ct-DNA and provide some useful data for the effect of resorcinol on human diseases.