R. G. Ingalls, R. G. Squires, L. Butler
Nov 1, 1975
Citations
0
Influential Citations
57
Citations
Journal
Biotechnology and Bioengineering
Abstract
Immobilized chymotrypsin catalyzes esterification of N‐acetyltyrosine in a medium containing high concentrations of alcohols. The hydrophilic support and inclusion of glycerol protect the enzyme activity and allow catalysis to proceed in the presence of only 10% (v/v) water. The same equilibrium concentration of ester is obtained whether reaction proceeds from ester or from free acid. Hates of ester synthesis and hydrolysis are similar when measured under the same conditions, but are at least one order of magnitude slower than optimal rates of hydrolysis. Subtilisin Carlsberg in the free, unmodified form catalyzes ester synthesis at even lower water concentrations; optimal rates are obtained at 5–15% H2O.