S. Gale, D. Burritt, S. Adams
Oct 1, 2016
Citations
1
Influential Citations
12
Citations
Journal
Aquaculture
Abstract
Abstract The purpose of this study was to determine the effects of adding ethylenediaminetetraacetic acid (EDTA) into the fertilization (50 μM) and embryo incubation (25 μM) seawater of Perna canaliculus. With EDTA, 78.6% of oocytes developed to D-larvae in comparison to 1.2% without EDTA (P Statement of relevance EDTA is routinely used in many shellfish hatcheries to reduce possible metal toxicity. However, the underlying biological mechanisms associated with EDTA use are poorly understood. We investigate the biological impacts of EDTA on P. canaliculus embryos and show for the first time that without EDTA embryos show oxidative stress and ultrastructure abnormalities. This study adds significantly to our understanding of the beneficial role(s) of EDTA in aquaculture.