Yan Ye-xia
2012
Citations
0
Influential Citations
0
Citations
Journal
Central South Pharmacy
Abstract
Objective To establish an RP-HPLC method for the determination of 5-O-methylvisammioside in Fangfeng Tongsheng Wan.Methods A XTerra C18(4.6 mm×250 mm,5 μm)column was used.The mobile phase was methanol-water=35∶65,the detective wavelength was 254 nm,the flow rate was 1.0 mL·min-1,and the column temperature was 28 ℃.Results The linear range of 5-O-methylvisammioside was 0.027 06-0.297 7 μg,r=0.999 7,and the average recovery was 99.7% with RSD=1.5%.Conclusion This method is repeatable,flexible,and highly sensitive.It can be used for the quality control of Fangfeng Tongsheng Wan.