Rushia A. Turner, Kinneret Shefer, M. Ares
Dec 1, 2013
Citations
0
Influential Citations
29
Citations
Journal
RNA
Abstract
1-methyl-7-nitroisatoic anhydride (1m7) is a useful reagent for probing RNA structure and serves as the basis for SHAPE analysis. This paper reports a reliable safe and inexpensive method for converting the precursor (4NIA) to 1m7. This method may make SHAPE analysis available to most molecular biology laboratories.