R. Wilson, L. Fulton
Oct 1, 2011
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Journal
Journal of biomolecular techniques
Abstract
The Genome Center (GC) at Washington University School of Medicine has developed a state of the art genomics facility. Our scientists work on a variety of cutting edge projects with researchers from around the world. These collaborative research projects lead to cutting edge advances in the field of genomics. The structural organization at the GC reflects these efforts and is centered around six major scientific areas: Transcriptome Sequencing, Genome Assembly, Whole Genome Sequencing, Human Microbiome, Human Genetics, and Targeted Resequencing. These specific scientific areas are supported by one central data production pipeline. Attributes of this pipeline include detailed sample screening protocols, sample barcoding capabilities that allow for a broad range of sample cohorts, multiplatform data production, and the ability to select from more than one method of sequencing strategies. All of this is supported by one centralized LIMS group dedicated to maintaining and developing the data production capabilities. The technology development group investigates new techniques and instrumentation prior to any changes in the main data production pipeline. Only robust protocols and instrumentation are allowed into the data production pipeline. This strategy allows The Genome Center to run a base data production pipeline while constantly infusing high quality advances. Sequence data for each project is sent into an advanced analysis pipeline built to conduct a multitude of assessments. When needed, validation (a second sequence event) can be used to confirm variants detected by the analysis software.