M. Nagao, T. Sugimura
Nov 1, 1972
Citations
0
Influential Citations
36
Citations
Journal
Cancer research
Abstract
The chemical carcinogens, 4-nitroquinoline 1-oxide and 4-nitropyridine 1-oxide, and their carcinogenic or noncarcinogenic derivatives were tested by a slight modification of the rapid screening method for mutagens and carcinogens described by Slater, Anderson, and Rosenkranz. N -Methyl- N′ -nitro- N -nitrosoguanidine and methylmethanesulfonate were also tested. 4-Nitroquinoline 1-oxide, 4-hydroxyaminoquinoline 1-oxide, 3-methyl-4-nitropyridine 1-oxide, and 2,3-dimethyl-4-nitropyridine 1-oxide caused wider growth-inhibiting zones with mutants of Escherichia coli , such as the DNA polymerase I-deficient mutant, pol A1, and the recombination-deficient mutants, rec A13 and rec B21, than with the wild strain. These compounds also caused wider growth-inhibiting zones with ultraviolet-sensitive mutants of Saccharomyces cerevisiae and Bacillus subtilis than with the corresponding wild types. 4-Nitropyridine 1-oxide, N -methyl- N′ -nitro- N -nitrosoguanidine, and methylmethanesulfonate caused wider growth inhibition zones with the pol A1, rec A13, and rec B21 mutants of E. coli than with the wild strain but similar zones with ultraviolet-sensitive mutants of S. cerevisiae and B. subtilis and the wild strains. The results indicate that different mechanisms are involved in repair of DNA damaged by ultraviolet irradiation or 4-nitroquinoline 1-oxide and its derivatives on the one hand and by 4-nitropyridine 1-oxide, N -methyl- N′ -nitro- N -nitrosoguanidine, or methylmethanesulfonate on the other.