D. F. Meigh
Oct 1, 1952
Citations
0
Influential Citations
48
Citations
Journal
Nature
Abstract
IT is possible to separate the dinitrophenyl-hydrazones of keto-acids by paper partition chromatography with the usual two-phase alcohol–water solvents1,2. Aldehydes and ketones without a carboxyl group are not appreciably separated by this means because, being less soluble in aqueous media, they have uniformly high RF values. Rice, Keller and Kirchner3 were able to separate a number of these compounds by using single-phase solvents such as diethyl ether–petroleum ether. They also found that filter paper impregnated with silicic acid was helpful. Kostir and Siavik4 used paper on which the solvent phases were reversed. They acetylated strips of filter paper and used two-phase solvents such as ethanol-chlorobenzene, the ethanol phase being the mobile one. In this way they separated acetaldehyde, formaldehyde, acetone and a number of keto-acids and dicarbonyl compounds.