Ya-ru Song, L. Niu, De-fa Wang
Nov 1, 2003
Citations
0
Influential Citations
9
Citations
Journal
IEEE Journal of Solid-state Circuits
Abstract
A simple and sensitive high-performance liquid chromatographic procedure for the determination of the trans isomer of glimepiride is reported. Chromatography accomplished direct separation of the cis and trans isomers of glimepiride on a Dikmonsil C18 (250 x 4.6 mm, 5 μm) column with a mobile phase consisting of methanol-acetonitrile-NH 4 Ac buffer solution (1.5 mol L -1 , pH = 4.5) (1.1:1.3:1.0, v/v) at a flow rate 0.5 mL min -1 . The resolution (R S ) was 1.73 with a retention time of 24.885 and 23.018 min for the cis and the trans isomer, respectively. A standard linear calibration curve was established for the trans isomer of glimepiride over the range of 4.95-198.00 μg mL -1 with a correlation coefficient of 0.99997. This method has been successfully used to analyze four different kinds of glimepiride product.