Su Ming-wei
2013
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Journal
Guangzhou Chemical Industry
Abstract
A new method was developed for the separation of the five amino acids(glutamine,arginine,alanine, valine,and phenylalanine) by precolumn derivatization with 9 - fluorine acid methyl ester(FMOC) by reversed phase high performance liquid chromatography.Under the optimized chromatographic conditions,five amino acids were completely separated.In the range of 0.025 ~ 0.2 mmol/L,the linear calibration for five amino acids was from 0.9920 to 0.9979.