J. Cremer, H. Meyer, K. Henning
Oct 11, 1991
Citations
0
Influential Citations
8
Citations
Journal
Journal of Chromatography A
Abstract
Abstract Cyanoginosins produced by Microcystis aeruginosa sp. are toxic cyclic heptapeptides with molecular weights of about 1000 dalton. Microcystis aeruginosa strain PCC 7806 proved to synthesize a methylated and non-methylated cyanoginosin-LR. A reversed-phase medium-pressure liquid cromatographic method was developed to separate the two cyanoginosin homologues on a preparative scale. The influence of chromatographic conditions on the separation such as solvent system, gradient parameters, flow-rate and temperature was investigated systematically. After optimization, up to 1.5 mg of the two compounds could be separated completely within 40 min.