J. Venable, R. Coggeshall
May 1, 1965
Citations
53
Influential Citations
4,599
Citations
Quality indicators
Journal
The Journal of Cell Biology
Abstract
A disadvantage of the lead strains used in electron microscopy is the amount of time required for their preparation. One of the more stable and reliable stains, commonly called the lead citrate stain or Reynolds' stain (Reynolds, 1963), is made by mixing lead nitrate and sodium citrate in distilled water, allowing time for lead citrate to form, then adding sodium hydroxide to raise the pH of the solution to 12. This communication reports the use of a commercially available lead citratO to eliminate the