A. Deiters, T. Cropp, D. Summerer
Dec 6, 2004
Citations
5
Influential Citations
238
Citations
Quality indicators
Journal
Bioorganic & medicinal chemistry letters
Abstract
Here, we report a generally applicable PEGylation methodology based on the site-specific incorporation of para-azidophenylalanine into proteins in yeast. The azido group was used in a mild [3+2] cycloaddition reaction with an alkyne derivatized PEG reagent to afford selectively PEGylated protein. This strategy should be useful for the generation of selectively PEGylated proteins for therapeutic applications.