B. Johnson-Wint, S. Hollis
May 15, 1982
Citations
4
Influential Citations
111
Citations
Quality indicators
Journal
Analytical biochemistry
Abstract
Abstract The fluorescence DNA assay for cells in culture or in tissue fragments described here is simpler than other 3,5-diaminobenzoic acid methods of determining cell number since it requires minimum handling of cells prior to measurement of their DNA. In this method 3,5-diaminobenzoic acid is reacted with DNA in situ on fixed dried films of cells or tissue. The method is sensitive, precise, and accurate.