Shuwei Li
Dec 16, 2011
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0
Influential Citations
2
Citations
Journal
ChemBioChem
Abstract
Do not disturb! As an environment-sensitive fluorescent amino acid, L-(7-hydroxycoumarin-4-yl) ethylglycine (7HC) is genetically encodable in E. coli containing a suppressor tRNA and a cognate 7HC-specific tRNA synthetase. Its incorporation into a full-length protein allows the detection of a nearby phosphorylation event with minimum influence on protein structures and functions, thereby serving as the smallest phosphorylation sensor.